CheV enhances the virulence of Salmonella Enteritidis, and the Chev-deleted Salmonella vaccine provides immunity in mice

Background Salmonella enteritidis (SE) is a major zoonotic pathogen and causes infections in a variety of hosts. The development of novel vaccines for SE is necessary to eradicate this pathogen. Genetically engineered attenuated live vaccines are more immunogenic and safer. Thus, to develop a live attenuated Salmonella vaccine, we constructed a cheV gene deletion strain of SE (named ΔcheV) and investigated the role of cheV in the virulence of SE. First, the ability to resist environmental stress in vitro, biofilm formation capacity, drug resistance and motility of ΔcheV were analyzed. Secondly, the bacterial adhesion, invasion, intracellular survival assays were performed by cell model. Using a mouse infection model, an in vivo virulence assessment was conducted. To further evaluate the mechanisms implicated by the reduced virulence, qPCR analysis was utilized to examine the expression of the strain’s major virulence genes. Finally, the immune protection rate of ΔcheV was evaluated using a mouse model. Results Compared to C50336, the ΔcheV had significantly reduced survival ability under acidic, alkaline and thermal stress conditions, but there was no significant difference in survival under oxidative stress conditions. There was also no significant change in biofilm formation ability, drug resistance and motility. It was found that the adhesion ability of ΔcheV to Caco-2 cells remained unchanged, but the invasion ability and survival rate in RAW264.7 cells were significantly reduced. The challenge assay results showed that the LD50 values of C50336 and ΔcheV were 6.3 × 105 CFU and 1.25 × 107 CFU, respectively. After the deletion of the cheV gene, the expression levels of fimD, flgG, csgA, csgD, hflK, lrp, sipA, sipB, pipB, invH, mgtC, sodC, rfbH, xthA and mrr1 genes were significantly reduced. The live attenuated ΔcheV provided 100% protection in mice against SE infection. Conclusion All the results confirmed that the deletion of the cheV gene reduces the virulence of SE and provides significant immune protection in mice, indicating that ΔcheV could be potential candidates to be explored as live-attenuated vaccines. Supplementary Information The online version contains supplementary material available at 10.1186/s12917-024-03951-x.


Background
Salmonella Enteritidis (SE) ranks as a major zoonotic pathogen, prevalent in both livestock, poultry wildlife and humans.It leads to considerable economic losses in animal agriculture and is responsible for numerous human fatalities.This pathogen poses a severe threat to both economic progress and public health [1].SE infection in humans is mainly transmitted through the environment, animal carriers, and water.Poultry and livestock, as food-producing animals, are considered the primary sources of human SE infections [2].
For many years, using antibiotics was the primary method for controlling Salmonella infection.However, the development of multiple antibiotic resistance mechanisms in bacteria has led to a reevaluation of antibiotic use.Currently, vaccination is considered the most effective and cost-efficient method to reduce or control Salmonella infection [3].Salmonella vaccines commercially available are typically classified into three main types: live attenuated vaccines, inactivated vaccines, and subunit vaccines.The immunological effects of these vaccines vary significantly, as established by numerous studies.While inactivated vaccines are known for their safety and ability to elicit high antibody levels, they often fall short in providing adequate immune protection.This shortfall is attributed to their lack of ability to induce robust cellmediated immunity, which is essential for the eradication of intracellular pathogens like Salmonella [4,5].On the other hand, subunit vaccines are also safe but usually necessitate multiple doses to extend the duration of immunity, and require suitable adjuvants to elicit a potent cellular immune response.Genetically the live attenuated vaccines, created by knocking out virulence genes, can induce strong cell-mediated immunity, because the vaccine strain can invade the host and present nearly all antigenic components of the bacteria [6][7][8].Identifying virulence genes is key to developing gene deletion live vaccines, and many researchers have already developed a variety of vaccines using this method, including for Brucella, Salmonella Typhimurium, Yersinia pestis, Vibrio anguillarum, and Edwardsiella tarda [9][10][11][12][13].
SE spreads through the fecal-oral route and, to successfully colonize the host, must overcome the acidic stress of the gastrointestinal tract, adhere to the intestinal mucosa, and disrupt epithelial cells.It can also penetrate beneath the mucosa to be phagocytosed by macrophages.As an intracellular parasite, SE has evolved remarkable capabilities to resist intracellular bactericidal components.It then spreads throughout the body via the bloodstream or lymphatic system, carried by macrophages, leading to systemic infection [14].The chemotaxis allows Salmonella to perceive changes in the external environment within host tissues, such as pH, osmotic pressure and temperature, and evade host immune pressure, overcome colonization resistance and resist antimicrobial treatment by regulating the expression of specific genes, thus surviving and colonizing within the host.Additionally, the chemotaxis also controls drug resistance, biofilm formation and virulence [15][16][17][18].Researches indicate that CheV is a crucial chemotactic protein closely associated with various pathogenic processes.These include the adhesion and chemotaxis in Vibrio cholerae, chemotaxis in Bacillus subtilis, biofilm formation and motility in Campylobacter jejuni and Vibrio anguillarum, the adhesion, chemotaxis, and motility of Vibrio harveyi, as well as the colonization and infection processes in Helicobacter pylori [19][20][21][22].Genomic analysis has revealed that Salmonella Enteritidis (SE) carries the cheV gene.Researchers using differential proteomics analysis on Salmonella Typhimurium found that CheV expression decreases after infecting epithelial cells, suggesting that this gene may influence the virulence of SE through multiple mechanisms [23].
Based on these studies, we hypothesize that the cheV gene plays an important role in SE's biofilm formation, adhesion, invasion ability and virulence.To test this hypothesis, we constructed a cheV gene deletion strain using homologous recombination technology and analyzed the impact of the cheV gene on the virulence of SE through in vitro stress simulation tests, detection of biofilm formation ability, drug resistance, motility, adhesion, invasion, intracellular survival assays, LD 50 determination and virulence gene expression analysis.Additionally, to demonstrate the feasibility of using the cheV gene deletion strain as a vaccine against Salmonella, we evaluated the immune protection rate of ΔcheV using a mouse model.These study results provide an important theoretical basis for understanding the impact of the cheV gene on SE's virulence.

Bacterial strains, cells and plasmids
Bacterial strains and plasmids used in this study are listed in Table 1.Salmonella enteritidis C50336 was maintained in the Key Laboratory of Preventive Veterinary Medicine,

Construction of the cheV gene deletion strain
The cheV gene deletion strain was constructed using the λ-Red recombination technique [24].The C50336 strain carrying the pKD46 plasmid was cultured in LBA containing 225 mg/mL L-arabinose at 30 °C until its OD 600 nm reached 0.6-0.8.It was then washed 3 times with precooled autoclaved ultrapure water and 10% glycerol to prepare competent cells.Next, using the pKD3 plasmid as a template, specific primers P1, P2 (Table 2) were used to amplify homologous fragments.The purified fragments were electroporated into C50336 (pKD46) competent cells, followed by the addition of 1 mL of LB liquid medium and incubation at 30 °C for 2 h.The culture was then spread on LB agar containing 50 µg/mL chloramphenicol (Cm).Colonies that grew were identified using specific primers P3, P4 (Table 2).Positive strains, after removal of the pKD46 plasmid at 42 °C, were named ΔcheV::cat.To remove the Cm segment, the pCP20 plasmid was electroporated into ΔcheV::cat, following the same process, and mutants were identified using specific primers P3, P4.The positive strains, after removal of the pCP20 plasmid at 42 °C, were named ΔcheV.
To generate the ΔcheV-complemented strain, polymerase chain reaction (PCR) was performed with the primers P5, P6 (Table 2) to amplify the cheV open reading frame.The purified PCR product was then chemically transformed into PMD-19T vector.The PMD-19T-cheV was electroporated into ΔcheV and P5, P6 was used to confirm the complemented strain.The positive strains were named ΔcheV + cheV.

In vitro stress simulation assay
The bacterial culture during the logarithmic phase was washed 3 times with sterilized saline, and then serially diluted for counting, to determine the original bacterial count.The culture was incubated separately in strong acid solution (pH 3.5), strong alkaline solution (pH 10), at 42 °C for 1 h and in an oxidative stress solution (10 mmol/L H 2 O 2 ) for 10 min.The bacteria were counted again after these stress treatments to determine the poststress bacterial count.The survival rate was calculated as the post-stress bacterial count divided by the original bacterial count.

Detection of biofilm formation
Referring to previous research methods, the ability to form biofilms was assessed using the crystal violet (CV) staining method [25].C50336, ΔcheV and ΔcheV + cheV were inoculated into 6 mL of LB liquid medium (ΔcheV + cheV was in LBA) and incubated statically at 28 °C for 3 days.The biofilms were washed with PBS, fixed in absolute methanol for 15 min, and stained with 2% CV for 15 min to observe the thickness and staining of the bacterial ring.The quantitative detection method is as follows: 150 µL of bacterial suspension was added to each well of a 96-well plate, stained as above, and finally, 200 µL of absolute ethanol was added to each well to dissolve the CV.The absorbance was measured at 570 nm, and the assay was repeated 3 times.
We also studied the effect of the cheV gene on the main components of biofilms: curli and cellulose formation.Referring to previous methods [26], 5 µL of overnightcultured bacterial suspension was inoculated onto LB agar containing 160 mg/L Congo red and 10 mg/L Coomassie brilliant blue without salt, incubated at 28 °C for 2 days, and the colony morphology and color were observed to assess the production of curli.Similarly, 5 µL of overnight-cultured bacterial suspension was inoculated onto LB agar without salt containing Calcofluor White Stain (200 mg/L) and incubated at 28 °C for 2 days.The production of cellulose was evaluated by observing the fluorescence intensity under ultraviolet (UV) light (366 nm).

Detection of drug resistance
The sensitivity of ΔcheV to antibiotics was tested using the Kirby-Bauer (K-B) method.According to the standards of the United States National Committee for Clinical Laboratory Standards (NCCLS), 17 drugs were selected for testing.These drugs include: ampicillin, piperacillin, amoxicillin, cefazolin, cephalothin, cefoperazone, cefuroxime, ceftriaxone, gentamicin, amikacin, kanamycin, streptomycin, tetracycline, doxycycline, minocycline, ciprofloxacin and levofloxacin.Overnightcultured bacterial suspension was evenly spread on LB agar plates.Commercial antibiotic susceptibility disks were placed on the inoculated plates using tweezers.The plates were then incubated at 37 °C for 12 h, and the diameters of the inhibition zones were measured.The results were interpreted according to the NCCLS antibiotic susceptibility testing standards.

Motility assay
Referring to previous methods [27,28], 5 µL of overnightcultured bacterial suspension was inoculated onto LB semi-solid agar plates (0.3% agar).The plates were then incubated at 37 °C for 5-6 h.The diameter of the swimming halo was measured, and the assay was repeated 3 times.

Adhesion, invasion and intracellular survival assays
As previously described [9,25], Caco-2 cells were seeded at a density of 1 × 10 5 cells per well in a 12-well plate.C50336, ΔcheV and ΔcheV + cheV were resuspended in PBS for counting and added to the cells with a multiplicity of infection (MOI) of 100.The mixture was centrifuged at 1000 rpm for 5 min and then incubated in a 37 °C, 5% CO 2 incubator for 1 h.The cells were lysed with 1% Triton X-100 for 8 min, and the lysates were serially diluted for counting.For the invasion assay, after infecting the cells as described above, the cells were further incubated in DMEM containing gentamicin (100 µg/mL) for 1 h to remove extracellular bacteria.The cells were then lysed with 1% Triton X-100 and counted.Adhesion rate = (number of adherent bacteria) / (number of infecting bacteria per well) × 100%; Invasion rate = (number of invaded bacteria) / (number of infecting bacteria per well) × 100%.
For the intracellular survival assay, as previously described [25,28,29], RAW264.7 cells were seeded at a density of 10 5 cells per well in 2-12 well plates.C50336, ΔcheV and ΔcheV + cheV infected the cells with an MOI of 100 and were incubated in a 37 °C, 5% CO 2 incubator for 2 h.The cells were then washed twice with PBS to remove non-adherent/invasive bacteria.Subsequently, the cells were incubated in DMEM containing gentamicin (100 µg/mL) for 1 h to eliminate all remaining extracellular bacteria.The cells were lysed with 1% Triton X-100 and counted as the 3-h intracellular bacterial count.Cells in the other plate were incubated in DMEM containing 10 µg/mL gentamicin for 20 h, lysed with 1% Triton X-100, and counted as the 23-h intracellular bacterial count.Intracellular survival rate = (number of intracellular bacteria at 23 h) / (number of intracellular bacteria at 3 h) × 100%.

Determination of LD 50 in mice
Referring to previous methods [9], 55 female Kunming mice, aged 4-6 weeks, were randomly divided into 11 groups (n = 5).The first five groups were intraperitoneally (i.p.) injected with C50336 at doses ranging from 2 × 10 7 to 2 × 10 3 CFU/mouse.The next five groups were i.p. injected with ΔcheV at doses ranging from 2.5 × 10 9 to 2.5 × 10 5 CFU/mouse.The remaining group was i.p. injected with an equal volume of PBS.The death of mice was observed and recorded over a period of 14 days.The LD 50 value was calculated using the formula of log

RNA extraction and quantitative real-time PCR (qPCR)
To further investigate the role of the cheV gene in the virulence of SE, we employed qPCR technology to detect the expression levels of virulence genes.RNA was extracted using a bacterial RNA extraction kit (Beijing Aidlab Biotechnologies Co., Ltd., China), and DNA was removed through DNase I treatment.Then, RNA was reverse-transcribed into cDNA using a reverse transcription kit (Bohang Biotechnology Co., Ltd., China).Using this cDNA as a template and based on literature [31,32], primers were designed (Table 3).qPCR detection was performed using the SYBR Green dye method.

Immunization of ΔcheV via i.p. route
Referring to previous methods [8], 30 female Kunming mice, aged 6-8 weeks, were randomly divided into 3 groups (n = 10), namely the immune group (Group A), the C50336 infection group (Group B) and the PBS control group (Group C).Group A was i.p. injected with a dose of 2.5 × 10 6 CFU/mouse of ΔcheV (once on day 0 and once on day 14), while Group B and C were i.p. injected with an equal volume of PBS.45 days after the second immunization, mice in Groups A and B were simultaneously i.p. injected with a dose of 2 × 10 7 CFU/mouse of C50336, and Group C was injected with an equal volume of PBS.The survival of mice was recorded daily for 14 days post-infection (dpi).The immune protection rate of ΔcheV to mice can be calculated according to the formula: (mortality rate of the control group -mortality rate of the immune group)/mortality rate of the control group × 100%.

Statistical analysis
Statistical analyses were conducted using GraphPad Prism v 8.0, employing one-way Analysis of Variance (ANOVA) followed by t-tests.Data are presented as mean ± standard error.Significant differences are indicated with an asterisk (*), where *p < 0.05, **p < 0.01, ***p < 0.001 are considered to represent statistically significant differences in mean values.

Results
The cheV gene affects the resistance of SE to environmental stress Using λ-Red recombination technology, a cheV gene deletion mutant was constructed in C50336.As shown in Fig. 1A, the deletion strain of the cheV gene and the complementation strain were successfully constructed.
To study whether the cheV gene influences the resistance of SE to various environmental stresses, we compared the survival of C50336 and ΔcheV in strong acid stress solution, strong alkaline stress solution, at 42 °C and in oxidative stress solution.The results (Fig. 1B) showed that, compared to C50336, the survival rate of the ΔcheV significantly decreased in acidic, alkaline and thermal environments.There was no significant change in survival rate under oxidative stress conditions.This indicates that the cheV gene plays a role in affecting the resistance of SE to acid, alkaline and thermal stresses.

The cheV gene does not affect biofilm formation ability and drug resistance of SE
Biofilm formation assays were conducted for C50336, ΔcheV and ΔcheV + cheV.The results showed that in test tubes, C50336, ΔcheV and ΔcheV + cheV were all capable of forming biofilms (Fig. 2A), with no significant differences in thickness and color intensity.Quantitative results revealed that the biofilms formed by the 3 strains had similar OD 570 nm values after staining and dissolution (Fig. 2B).Curli detection indicated that all 3 strains formed red, rough colonies (Fig. 2C), suggesting that all strains could produce curli.The cellulose detection results showed that the colonies of all 3 strains emitted the same fluorescence intensity under UV light (Fig. 2D).These findings suggest that the cheV gene does not affect biofilm formation in SE.
Drug susceptibility tests were conducted using the K-B method, and the results were interpreted according to the standards of the NCCLS.The results (Table 4) showed that compared to the C50336, ΔcheV only exhibited increased resistance to cefoperazone.There were no significant differences in sensitivity to other antibiotics.This indicates that the cheV gene has a minimal impact on the drug resistance of SE.

The cheV gene does not affect the motility of SE
Using 0.3% semi-solid agar for motility testing, the results showed that the average diameters of motility for the C50336, ΔcheV and ΔcheV + cheV strains were 25 mm, 24 and 25 mm respectively (Fig. 3).There was no significant difference among the 3 strains, indicating that the deletion of the cheV gene does not affect the motility of SE.The cheV gene affects the invasion and intracellular survival ability of SE Using Caco-2 and RAW264.7 cell models, the adhesion, invasion and intracellular survival abilities of the ΔcheV were tested (Fig. 4).The results showed that the adhesion ability of the ΔcheV to Caco-2 cells was not significantly different compared to C50336.However, its invasion ability and intracellular survival ability significantly decreased.These results indicate that the deletion of the cheV gene does not affect the adhesion ability of SE but does reduce its invasion and intracellular survival capabilities.

Deletion of the cheV gene attenuates the virulence of SE
Mice were infected with C50336 and ΔcheV via the i.p. route.Both groups began to exhibit mortality on the 3rd dpi, showing typical symptoms such as trembling, arched backs, crusted eyes and disheveled fur.In contrast, the control group mice had smooth fur and good mental state.The LD 50 calculation results (Table 5) showed that the LD 50 for C50336 and ΔcheV were 6.3 × 10 5 CFU/ mouse and 1.25 × 10 7 CFU/mouse, respectively.This indicates that the LD 50 of ΔcheV is approximately 20 times higher than that of C50336 (1.25 × 10 7 /6.3 × 10 5 ≈ 20), suggesting that the deletion of the cheV gene reduces the virulence of SE.

Reduced expression levels of virulence genes in cheV gene deletion strain
To further investigate the impact of the cheV gene on the virulence of SE, the expression levels of various virulence genes following the deletion of the cheV gene were examined using the qPCR method.The results (Fig. 5) showed significant reductions in the expression levels of fimD, flgG, csgA, csgD, hflK,lrp, sipA, sipB, pipB, invH, mgtC, sodC, rfbH, xthA, and mrr1.This indicates that the deletion of the cheV gene leads to reduced expression of multiple virulence genes in SE.
The cheV gene deletion strain provides good immune protection in mice 14 days after the final immunization, mice in Group A and Group B were simultaneously infected with C50336 through the i.p. route, while Group C was again injected with PBS.The results (Fig. 6) showed that both Group A and Group C mice demonstrated a 100% survival rate over 14 days, with none of the mice exhibiting typical clinical symptoms of SE infection.In contrast, the mortality rate in Group B was 90% within the same period, and typical symptoms of SE infection, such as trembling, arched backs, crusted eyes and disheveled fur, were observed in these mice.According to the formula for calculating immune protection rate, the immune protection  rate of ΔcheV is 100%.This indicates that ΔcheV provided 100% protection in mice against SE infection.

Discussion
In the process of developing genetically engineered attenuated live vaccines, selecting suitable candidate genes for attenuation is crucial.Chemotaxis affects the survival and colonization abilities of Salmonella within the host, which in turn impacts the bacterium's virulence [31,33].The CheV, as a coupling protein in the chemotaxis system, might influence the virulence of SE.To confirm this hypothesis, we constructed a cheV gene deletion strain.
Both in vivo and in vitro assays, it was found that compared to the C50336, the cheV gene deletion strain exhibited significantly reduced virulence, indicating that the cheV gene plays an important role in the virulence of SE.
In the process of pathogenesis, SE must contend with many adverse environmental conditions, including antimicrobial peptides, changes in temperature and pH, and limited availability of nutrients [34].Additionally, the host's inflammatory response, which recruits phagocytic cells, exposes Salmonella to other unfavorable conditions such as oxidative and nitrosative stress [35].The ability to survive under different environmental stress conditions is a fundamental characteristic of SE's pathogenicity [36].In this study, we found that the ΔcheV, compared to the wild type, showed a significant decrease in survival rate under acid, alkaline, and thermal stress.This suggests that the deletion of the cheV gene weakens SE's perception of acid, alkaline, and thermal environments, preventing it from making appropriate responses to avoid and endure stressful conditions, thereby reducing its survival rate and affecting its pathogenic process.
Moreover, biofilm formation is one of Salmonella's adaptive mechanisms for surviving in adverse conditions.It protects the bacteria from harsh external environments, enhancing SE's resistance to conditions such as dryness, extreme temperatures, antibiotics and preservatives [34,37].In this study, the biofilm formation ability of SE after the deletion of the cheV gene was investigated.It was found that there was no significant difference in the biofilm formation of ΔcheV compared to C50336, and the production of curli and cellulose was consistent with that of C50336 and ΔcheV + cheV.qPCR analysis revealed that the expression levels of the biofilm formation-related genes csgA and csgD were significantly reduced.A possible explanation is that, although the deletion of the cheV gene led to a decrease in the expression levels of some genes related to biofilm formation, biofilm formation is regulated by multiple factors, and overall, the deletion of the cheV gene does not significantly affect the biofilm formation capability.Biofilm formation ability is often closely related to bacterial drug resistance [38].The drug resistance testing of the ΔcheV indicated that, compared to C50336, there was no significant change in its sensitivity to most antibiotics, which aligns with expectations.However, it is important to note that the parental strain C50336 is not highly resistant to antibiotics, which might mislead us to believe that the deletion of the cheV gene does not affect the bacterial drug resistance.
In the host body, the strength of motility plays a crucial role in whether Salmonella can reach specific sites.After entering the small intestine, Salmonella must traverse the mucosal layer and then adhere to the intestinal epithelial cells.Therefore, motility is a prerequisite for Salmonella to increase its chances of contact with intestinal epithelial cells [39].Research on different bacteria has found that the impact of cheV on motility is inconsistent.In this study, we found that the motility of the ΔcheV on semi-solid agar did not change significantly.qPCR analysis showed a decrease in the expression levels of Salmonella motility-related genes flgG and fimD, while the expression level of prot6E did not change.Although there is a decrease in the expression levels of genes related to motility, other compensatory mechanisms may exist, leading to the deletion of cheV not significantly affecting the motility of SE.
To further investigate the impact of the cheV gene on the virulence of SE, we used both cellular and animal models for evaluating virulence.The pathogenesis of Salmonella primarily involves adhesion to epithelial cells, invasion, and survival within macrophages.In this study, we found that deletion of the cheV gene did not significantly change the adhesion capability of SE to epithelial cells, possibly because the formation of SE biofilms and motility were not significantly affected, resulting in no significant difference in the amount of adhesion to epithelial cells.However, the ability to invade and the intracellular survival rate in macrophages significantly decreased.Moreover, qPCR analysis revealed that the expression levels of genes related to invasion ability (invH) and genes related to intracellular survival in macrophages (sipA, pipB, mgtC and sodC) were significantly reduced.These results suggest that deletion of the cheV gene reduces the invasion and intracellular survival abilities of SE, leading to attenuated virulence.LD 50 measurements in mice models for C50336 and ΔcheV showed that the LD 50 of ΔcheV increased by about 20 times, indicating that the cheV gene influences the virulence of SE.To further investigate the role of the cheV gene in SE virulence, qPCR was used to detect the expression of various virulence genes after cheV gene deletion.It was found that the expression levels of genes involved in metabolism (rfbH), genes related to exonuclease/endonuclease activity (xthA and mrr1), genes associated with cell membrane and cell wall integrity (hflK and lrp), and genes related to type III secretion system (T3SS) (sipB, including sipA, pipB) were significantly reduced.This suggests that the deletion of cheV can downregulate the expression of various virulence genes, thereby reducing the virulence of SE consistent with the LD 50 test results.To determine the immune protection rate, mice were immunized twice with ΔcheV, and it was found that this method of immunization could 100% resist the infection by the wild-type strain.This result indicates that the cheV gene deletion strain has a good immune protective effect on mice and has the potential to be a candidate for developing genetically engineered attenuated live vaccines.
Developing attenuated Salmonella live vaccines requires skillfully balancing attenuation with immunogenicity.It's essential to ensure that the bacterial virulence is appropriately reduced, while also stimulating immunity without causing disease [40].Therefore, we will next examine the distribution of bacteria in various organs to further assess the virulence level of the ΔcheV.Additionally, after immunizing mice, we will measure immune indicators such as IgG, IgA and cytokines to further analyze the immune protection of the mutant.
In conclusion, this study demonstrates that the deletion of the cheV gene reduces the virulence of SE. and the cheV gene deletion strain can provide effective immune protection in mice.These findings lay the groundwork for subsequent research and development of related genetically engineered attenuated live vaccines.

Fig. 1 (
Fig. 1 (A) PCR verification of the cheV gene deletion strain and ΔcheV-complemented strain.C50336 means the wild-type strain; ΔcheV::cat means a first recombination strain; ΔcheV means a second recombination strain, ΔcheV + cheV means ΔcheV-complemented strain.The PCR product of C50336 has a length of 924 bp, the product of ΔcheV::cat has a length of 1510 bp, the product of ΔcheV has a length of 493 bp and the product of ΔcheV + cheV has a length of 1008 bp.(B) The survival rate of ΔcheV under various environmental stresses.The data represents the average of 3 replicates (*p < 0.05, ***p < 0.001, ns means not significant)

Fig. 3 (Fig. 2 (
Fig.3 (A)The images shown are representatives of several independent assays.(A) The motility of the strains was evaluated on 0.3% agar plates, measured after 5 h of incubation.The data represents the average of 3 replicates (ns means not significant)

Fig. 4 (Fig. 6 Fig. 5
Fig. 4 (A) Adhesion and invasion of bacteria in Caco-2 cells.(B) Intracellular survival in RAW264.7 cells.The results of the adhesion and invasion assays are presented as a ratio to the C50336 (The results of C50336 were considered as 100%).The data represents the average of 3 replicates (***p < 0.001, ns means not significant)

Table 1
Bacterial strains and plasmids used in this study

Table 4
Antibiotic susceptibility test of cheV gene deletion strain

Table 5
LD 50 of C50336 and ΔcheV in KM mice